BIOASSAY OF INSULIN BIOASSAY OF D-TUBOCURARINE MERITS OF BIOASSAY DEMERITS OF BIOASSAY REFRENCES. is being done through. Bioassay, official method, adrenaline, digitalis, d-tubocurarine, heparin, insulin, oxytocin, gonadotropin, chorionic gonadotropin, urokinase, streptokinase. Second 4 x 4 latin square bioassay was performed using a series: with a The presence of d-tubocurarine was demonstrated in the Peruvian.

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Mean dose which bkoassay head drop of the test sample is compared with the mean dose of standard preparation. Suitable dose of d-tubocurarine is 0. Two similar contractions with the same concentration of acetylcholine are obtained.

The potency of test sample is calculated from the standard curve. Numerous small segmental contribution coming from lower inter costal arteries. Rabbits recover immediately d-tublcurarine the effect of biosssay. Go to Application Have a question?

Animals should be free from disease, obtained from a healthy colony and should be accustomed with the experimental procedure. First group will receive standard sample and the second group will receive the sample under test. Newer Post Older Post Home. Acetylcholine contraction is recorded on slow moving drum for 90 sec.

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Use in Pharmacology The Rectus Muscle of frog is used in the screening of parasympatholytic agents. Those rabbits which received the standard sample on the first day will be given test bioasxay on the second day of expt. Plays an important role in breathing It helps to keep internal organ intact. In order to view it, please contact the author of the presentation.


Each rabbit is placed in a holder with its head protruding outside. You do not have the permission to view this presentation.

Upload from Desktop Single File Upload. Rabbits weighing 2 kg. Infusion is continued till the rabbit will not be in a position to hold its head erect or there will be no s-tubocurarine by focussing light on the eyes and the neck gets elongated and toneless. Conclusion Rectus abdominis muscle of frog has various importance in pharmacology. The total amount of test sample required to produce the endpoint is compared with the total amount of the standard sample required to produce similar endpoint.

PHARMACOLOGY: Bioassay of D-Tubocurarine

Blood Supply It has several sources for arterial blood supply. Location of Rectus muscle. A linear response will be obtained. I enjoy, result in I found exactly what I used to be looking for. They are divided into two groups each containing 4 rabbits. The muscle is stabilized for min. They mainly respond to the acetyicholine released from motor neuron terminal. D-tubpcurarine of D-Tubocurarine 1. Functions of Rectus muscle.


Functions It is an important postural muscle. College of Pharmacy, Ahmedabad.

The responses are recorded using isotonic frontal writing lever with 1 G. Share to Twitter Share to Facebook. WordPress Embed Customize Embed. Pharm 1 st semester Phamacology Under the Guidance of Mr. Head drop after injection. Have a nice day.


The presentation is successfully added In Your Favorites. Presentation Description No description available. Bioassay of D- Tubocurarine. The percentage reduction at each dose levels is calculated and log dose response curve of the standard drug is plotted.

God Bless you man. Minimum 8 rabbits are used. Screening Methods In Pharmacology. It is a perfect in vitro model. A frog is pithed and laid on its back on a cork covered board to which it is pinned. The head should be freely movable. Injection should be given at a rate of 0. Receptors present in Rectus muscle.

Rectus abdominis muscle is a paired muscle running vertically on each side of the anterior wall. D-tuocurarine The rectus abdominis muscle of frog mainly rich of Nicotinic receptor.

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